Nanostring nCounter

Arizona Genetics Core gives gene expression providers utilizing the NanoString nCounter® Evaluation System. This method makes use of molecular “barcodes” and single-molecule imaging to profile as much as 800 mRNAs, microRNAs, or DNA targets concurrently with excessive sensitivity and precision, with none reverse transcription or amplification.

Common Data

Pattern Enter

Gene Expression, miRNA and miRGE Assasys: • 100ng purified whole RNA or 300ng degradedCopy Quantity Variation: • 300ng purified genomic DNA

Arizona Genetics Core gives quite a lot of extraction providers for many pattern varieties, please inquire for particulars and pricing.

Anticipated Outcomes

Gene Expression Restrict of Detection: • 0.5fM spike-in management (~1 copy per cell); 90% of the time Fold-change Sensitivity: • >1.5 fold (>5 copies per cell) • >2 fold change (>1 copy per cell)miRNA Assay Restrict of Detection: • ≤ 0.5fM (~10 copies per cell) Fold-change Sensitivity: • > 2 fold changeCopy Quantity Variation Reproducibility: • 99% Variety of Copies Detected: • 0-4, multiallelicmiRGE Assays Limits of Detection: • For miRNA: >2.5 copies per cell copy per cell • For mRNA: 1 copy per cell Fold-change Sensitivity: • >2 fold change

Particulars

Pattern Preparation Particulars

Varies, please inquire.

Workflow

Quite a lot of pre-designed panels can be found for off-the-shelf use. Every cartridge can run as much as 12 samples, and most panel kits are bought with chemistry for 12, 24, 48 or 96 samples (Customized Panels begin at 48 samples). For a whole checklist of the variety of samples and particular genes lined by every panel, please go to the next hyperlinks:

Oncology Panels

  • Breast Most cancers 360™ Panel
  • PanCancer IO 360™ Panel
  • CAR-T Characterization Panel
  • Metabolic Pathways Panel
  • Hallmarks of Most cancers Assortment:
    • PanCancer Pathways Panel
    • PanCancer Immune Profiling Panel
    • PanCancer Development Panel
  • PlexSet™ Pre-selected Panels
  • Vantage 3D™ RNA Panels
  • Vantage 3D™ Gene Fusion Panels
  • miRNA Panels
  • nCounter Gene Fusion Panels (Ex-US solely)

Immunology Panels

  • Autoimmune Profiling Panel
  • Autoimmune Discovery Panel
  • Metabolic Pathways Panel
  • Fibrosis Panel
  • Human Organ Transplant Panel
  • Immunology Panels
  • Irritation Panels
  • miRNA Panels
  • Myeloid Panels
  • NHP Immunology Panel

Neuroscience Panels

  • Neuropathology Panels
  • Neuroinflammation Panels
  • Mouse and Human AD Panels
  • miRNA Panels
  • PlexSet™ Pre-selected Panels

Gene Fusion Panels and CNV Assays

Customized CodeSets For final flexibility, you possibly can work with NanoString immediately (session is freed from cost) to design a customized Gene Expression or Copy Quantity Variation code set tailor-made precisely to your analysis wants. That is particularly helpful for organisms that do not have already got pre-built panels, hard-to-target sequences, and splice variation and fusion transcript detection. The customized CodeSet design course of begins by choosing a listing of targets and submitting them to NanoString. As soon as the design is authorized by you, their Manufacturing Group will assemble your CodeSet, carry out in depth high quality management testing, and ship all elements on to the Arizona Genetics Core for processing. Customized CodeSet pricing is matrix based mostly, with the value per knowledge level declining with rising numbers of genes and numbers of samples assayed.miRGE Code Units Two assays, one reaction- Use this technique to detect subsets of each mRNAs and miRNAs throughout all organic ranges of expression. The nCounter miRGE Assay delivers correct and delicate profiling of 100 to 200 mRNA targets and between 5 and 30 miRNA targets, all in a single tube response.

Worth

Educational (UA): $575.00 | Educational(non-UA): $690.00 | Business: $862.50 | Unit: Cartridge

Be aware- Price per Cartridge doesn’t embrace QC, extra labor or NanoString Code Units. Reagents should be bought individually. Please inquire for extra particulars.

Turnaround Time

Is dependent upon assay kind; please inquire.

Associated Companies

Pattern Prep and QC

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